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2 article(s) from Rosemeyer, Helmut

Terminal lipophilization of a unique DNA dodecamer by various nucleolipid headgroups: Their incorporation into artificial lipid bilayers and hydrodynamic properties

Emma Werz and
Helmut Rosemeyer

Beilstein J. Org. Chem. 2015, 11, 913–929, doi:10.3762/bjoc.11.103

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Graphical Abstract

Figure 1: General structure of the fluorophore-labeled lipo-oligonucleotides and positions of lipophilization...

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Figure 2: Chemical formulae 1–9 and lipo-oligonucleotide sequences 10–15.

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Figure 3: Energy-minimized 3D structures of the lipophilic nucleoside headgroups 4a–9a. All 3D structures wer...

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Figure 4: Schematic display of the three main equilibria existing on the cis side of the bilayer chamber.

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Figure 5: Bilayer brightness vs event graph of 5’-d(4a-Cy5-TAG GTC AAT ACT) (10).

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Figure 6: Bilayer brightness vs event graph of 5’-d(5a-Cy5-TAG GTC AAT ACT) (11).

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Figure 7: Bilayer brightness vs event graph of 5’-d(6a-Cy5-TAG GTC AAT ACT) (12).

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Figure 8: Bilayer brightness vs event graph of 5’-d(7a-Cy5-TAG GTC AAT ACT) (13).

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Figure 9: Infiltration of lipo-oligonucleotide (13) aggregates from the buffer into the lipid bilayer. Z-dire...

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Figure 10: Bilayer brightness vs event graph of 5’-d(8a-Cy5-TAG GTC AAT ACT) (14).

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Figure 11: Bilayer brightness vs event graph of 5’-d(9a-Cy5-TAG GTC AAT ACT) (15).

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Figure 12: Summarizing the bilayer brightness for LONs 10–15 depending on the incubation time up to the maximu...

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Figure 13: Diffusion time measurements of LON 10 (5 µL, 50 nM). A) Z-direction scan directly after the additio...

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Figure 14: Hydrodynamic models of lipophilic oligonucleotides 10–15. Views are perpendicular (right) or parall...

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Figure 15: Scheme of a stretched rotation ellipsoid with the semi-major axes a and b.

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Figure 16: Schematic structure of a Cy5-labeled lipo-oligonucleotide.

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Figure 17: Fluorescence lifetime τ_F values of the LONs 10–15. A) Logarithmic imaging of fluorescence intensity...

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Full Research Paper

Published 01 Jun 2015

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Specific DNA duplex formation at an artificial lipid bilayer: fluorescence microscopy after Sybr Green I staining

Emma Werz and
Helmut Rosemeyer

Beilstein J. Org. Chem. 2014, 10, 2307–2321, doi:10.3762/bjoc.10.240

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Graphical Abstract

Figure 1: Chemical formulae and lipo-oligonucleotide sequences.

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Figure 2: Schematic illustrations (experiments A–F) of the specific DNA duplex formation at artificial lipid ...

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Figure 3: Experimental setup. Schematic drawing of the laser scanning microscope, the optical transparent mic...

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Figure 4: Chronological protocol of duplex formation of the lipo-oligonucleotide 4 with the complementary Cy5...

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Figure 5: Comparison of the bilayer brightness intensity with either Cy5 (irradiation: 635 nm) or Sybr Green ...

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Figure 6: Scheme of a z-scan of a lipid bilayer showing two locations for measurements of the diffusion times...

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Figure 7: Experiment B (4 + 6 + SG). Bilayer brightness as function of the various events (addition of oligon...

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Figure 8: Experiment D (4 + 9 + SG). Bilayer brightness as function of time and the various events [addition ...

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Figure 9: Conceivable geometry of the complex at the bilayer surface (cis compartment).

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Figure 10: Experiment E (10 + 6 + SG). Bilayer brightness as a function of time as well as of various events [...

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Figure 11: Experiment E (10 + 6 + SG). Bilayer brightness as function of the incubation time (A), of the perfu...

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Figure 12: Z-scans of the experiment E before and after the addition of Sybr Green I. A) Z-scan after the addi...

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Figure 13: Experiment E in reversed order of component addition; SG + 10 + 6.

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Figure 14: Bilayer brightness as a function of perfusion number and incubation periods for the experiment E in...

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Figure 15: Bilayer brightness as a function of perfusion number and incubation periods for the experiment F (SG...

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Figure 16: Kinetics of the tertiary complex formations of various lipo-DNA/DNA with Sybr Green I during incuba...

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Figure 17: Trafficking of a siRNA by a lipophilized DNA.

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Figure 18: A) Stage unit of the ‘Ionovation Explorer’ mounted on a standard inverted fluorescence microscope. ...

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Full Research Paper

Published 02 Oct 2014

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